DAILY PROGRAM

Day 1. Sunday,  June 15 Arrival and transfer to the hotel. Transfer to be arranged by each participant. 17:30 h. Meeting at the hotel lobby. 18:00-18:30 h. Presentation. 7^th Workshop Professor V. J. Benedí by Sebastián Albertí. 18:30-19:30 h. Lecture. What is new on the mechanisms of action and resistance to quinolones? by Jordi Vila. 20:30 h. First Official Dinner. Day 2. Monday,  June 16 08:30-09:30h Lecture. Why is antimicrobial resistance increasing and what can be done? by Reiner Helmuth. 09:45-10:15 h. Overview of the laboratory sessions. 10:15-10:30 h. Objectives of the day. 10:30-14:00 h. Laboratory session: T1: Disk diffusion and microdilution. T2: Preparation of agarose plugs for PFGE. T2: DNA extraction, REP-PCR and electrophoretic analysis. T5: Transference of ß-lactamase encoding plasmids by conjugation. 14:00-15:30 h. Lunch. 15:30-17:30 h. Laboratory session (cont.). 17:30-18:30 h. Lecture. Biofilms and antimicrobial resistance by Niels Hoiby. Day 3. Tuesday,  June 17 8:30-9:30 h. Lecture. PK / PD - approaches to suppression of resistance to anti-infectives by George L. Drusano. 09:45-10:15 h. Objectives of the day. 10:15-14:00 h. Laboratory session: T2: PFGE continued: restriction. T2: Staining and visualizing REP-PCR gel. T3: Isolation,electrophoretic analysis, preparation of competent cells and electroporation of plasmids encoding DNA repair genes. T5: Extraction for ß-lactamase assays (sonication). T5: Evaluation of transconjugants and assay of their antimicrobial susceptibility (E-test). T6: Extraction of outer membrane proteins. 14:00-15:30 h. Lunch. 15:30-17:30 h. Laboratory session (cont.) 17:30-18:30 h. Lecture. Community acquired antibiotic multirresistance: MRSA and ESBLs by Jesús Rodríguez-Baños. Day 4. Wednesday,  June 18 8:30-9:30 h. Lecture. Cations and susceptibility testing: Laboratory concerns by Luis Martínez-Martínez. 09:45-10:15 h. Objectives of the day. 10:15-14:00 h. Laboratory session: T2: PFGE continued: load and run gel. T3: Evaluation of electroporation results. T4: RNA extraction. T5: Antimicrobial susceptibility of transconjugants. T6: OMPs electrophoresis. 13:30-15:00 h. Lunch 15:00- 16:00 h. Lecture. Update on carbapenemases by Gian Maria Rossolini 16:00. Transfer to the hotel. Free afternoon. 21:00 h. Official dinner. Day 5.  Thursday,  June 19 09:45-10:15 h. Objectives of the day. 10:15-14:00 h. Laboratory session: T2: Interpretation of PFGE and REP-PCR. Discussion. T3: Test hypermutation. T5: Isoelectric focusing of ß-lactamase extracts. T5: Spectrophotometric assay of ß-lactamases. T6: OMPs electrophoresis and Western blot. 14:00-15:30 h. Lunch. 15:30-17:30 h. Laboratory session (cont.) 17:30-18:30 h. Lecture. Adaptive responses of Pseudomonas aeruginosa under antibiotic stress by Jesús Blázquez. Day 6.  Friday,  June 20 8:30-9:30 h. Lecture. Mechanisms and relevance of AmpC hyperproduction in P. aeruginosa by Antonio Oliver. 09:45-10:15 h. Objectives of the day. 10:15-14:00 h. Laboratory session: T3: Discussion of results of mutation frequency. T4: Analysis of mRNA expression. T5: PCR amplification of ß-lactamase genes. T6: Interpretation of SDS-PAGE and Western blot analysis of OMPs. 14:00-15:30 h. Lunch. 15:30-17:30 h. Laboratory session (cont.) 17:30-18:30 h. Lecture. Antimicrobial resistance genes and the environment: towards a new scenario? by Rafael Cantón Day 7. Saturday, June 21 8:30-9:30 h. Lecture Antimicrobial resistance in Group A Streptococcus by Sebastian Albertí. 09:45-10:15 h. Objectives of the day. 10:15-10:45 h. Laboratory session: T4: Evaluation of mRNA expression. 10:45-11:30 h. Questionnaire. Evaluation of the course. 1130-13:00. Visit to Valldemosa. Farewell Lunch: On-the-Beach.