Table of Contents
Nuevas estrategias para la identificaciÛn de aditivos y alimentos
Applications of analytical molecular biology methods. 1
Applications of analytical molecular biology methods. 2
DNA: the universal (biological) analyte
DNA and its building blocks
Examples of languages (messages)
DNA vs. amino acid information
DNA information is conserved
DNA sequences in public databanks (March 08, 2001)
The Polymerase Chain Reaction
Amplification step of the PCR
Technical variables and detection
Locust bean or guar? Molecular methods for detecting additions of guar gum to locust bean gum
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E 410 vs. E 412: what the EC says (I. Definitions) Off. J. of the E.C. L334, 09.12.98
E 410 vs. E 412: what the EC says (II. Identification) Off. J. of the E.C. L334, 09.12.98
E 410 vs. E 412: what the EC says (II. Identification)
E 410 vs. E 412: what the EC says (II. Identification)
Other methods from literature
Mannose to galactose ratios (I)
Mannose to galactose ratios (II)
Mannose to galactose ratios (III)
Lectin assays
Enzyme-Linked Lectin Assay (ELLA)
Development of new (DNA-based) methods
Seeds as sources of DNA
Unveiling the Markers sequences
Marker 1 restriction analysis
Marker 2 restriction analysis
Markers sequences
Carob vs. guar sequences (Marker 2)
Specific amplification of guar DNA from seeds
Investigation of DNA extraction from locust bean and guar gums (E 410 and E 412)
Specific amplification of guar DNA from mixtures of LBG and known additions of guar gum
Specific detection of guar DNA in commercial samples labeled as E 410
Specificity of the amplified products
PPT Slide
Molecular methods for detecting additions of guar gum to locust bean gum
Las nuevas nuevas tecnologÌas
Real-time PCR (quantitative)
The real-time PCR machine
SYBR Green fluorescent dye does not fluoresce until binding to double strand DNA
Upon binding primers, the dye starts to fluoresce
As more and more double strand DNA molecules (amplicons) are formed, more SYBR green molecules bind and fluorescence increases
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Author: vicente j. benedi
Email: vjbenedi@uib.es
Home Page: http://www.uib.es/depart/dba/microbiologia/VJBgrouphome.html
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