Real-time PCR (quantitative)
Notes:
In a conventional PCR, the number of amplicons increases as the number of cycles increases, but it is not until that a sufficient number of cycles have ben run that the amplicons can be detected (see top of the figure).
A conventional PCR is usually performed for 35-45 cycles and is basically an ìend-pointî analysis: two samples with two different amounts of target DNA to be amplified are PCR amplified for the same number of cycles and the compared by agarose gel electro
The real-time PCR machine provides real-time monitoring of the amount of new amplicons formed troughout every cycle. The reasons are: (1) a fluorecent dye is incorporated in the PCR mixture, and (2) the fluoresce in the mixture is read by buil-in fluoresc