PPT Slide

Different PCR curves are obtained for different amounts of initial DNA. End-points (plateaus) are obtained at different cycles: those samples with higheramounts of initial target DNA reach the plateau more rapidly

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Amplification from zero to one million copies of a fragment of the human b-globin gene. All samples contained SYBR Green I Dye. The reactions from one million to one thousand copies are easily distinguished. Below 1000 copies, the reactions begin to overl